HI 83200 Multiparameter Bench Photometer for Laboratories
Item Description: Brand New Hanna Instruments HI 83200 Multiparameter Bench Photometer for Laboratories
HI 83200 is a multiparameter bench photometer dedicated for Laboratory analysis. It can measure 45 different methods using specific liquid or powder reagents. The amount of reagent is precisely dosed to ensure maximum reproducibility. HI 83200 bench photometer can be connected to a PC via an USB cable. The optional HI 92000 Windows® Compatible Software helps users manage all their results
PRINCIPLE OF OPERATION
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices. If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical-chemical characteristics of substance according to the Lambert-Beer Law: -log I/Io = ελ c d or A = ελ c d
Where: -log I/Io=Absorbance (A) I o =intensity of incident light beam I =intensity of light beam after absorption ελ =molar extinction coefficient at wavelength λ c =molar concentration of the substance d =optical path through the substance Therefore, the concentration "c" can be calculated from the absorbance of the substance as the other factors are known. Photometric chemical analysis is based on the possibility to develop an absorbing compound from a specific chemical reaction between sample and reagents. Given that the absorption of a compound strictly depends on the wavelength of the incident light beam, a narrow spectral bandwidth should be selected as well as a proper central wavelength to optimize measurements. The optical system of HI 83200 is based on special subminiature tungsten lamps and narrow-band interference filters to guarantee both high performance and reliable results. Five measuring channels allow a wide range of tests. Instrument block diagram (optical layout) A microprocessor controlled special tungsten lamp emits radiation which is first optically conditioned and beamed to the sample contained in the cuvette. The optical path is fixed by the diameter of the cuvette. Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of intensity -Io- or -I-. The photoelectric cell collects the radiation -I- that is not absorbed by the sample and converts it into an electric current, producing a potential in the mV range. The microprocessor uses this potential to convert the incoming value into the desired measuring unit and to display it on the LCD. The measurement process is carried out in two phases: first the meter is zeroed and then the actual measurement is performed. The cuvette has a very important role because it is an optical element and thus requires particular attention. It is important that both the measurement and the calibration (zeroing) cuvette are optically identical to provide the same measurement conditions. Most of methods use the same cuvette for both, so it is important that measurements are taken at the same optical point. The instrument and the cuvette cap have special marks that must be aligned in order to obtain better reproducibility. The surface of the cuvette must be clean and not scratched. This is to avoid measurement interference due to unwanted reflection and absorption of light. It is recommended not to touch the cuvette walls with hands. Furthermore, in order to maintain the same conditions during the zeroing and the measurement phases, it is necessary to close the cuvette to prevent any contamination.
Light Life Life of the instrument Light Detector Silicon Photocell Environment 0 to 50°C (32 to 122°F); max 90% RH non-condensing Power Supply external 12 Vdc power adapter built-in rechargeable battery Dimensions 235 x 200 x 110 mm (9.2 x 7.87 x 4.33") Weight 0.9 Kg For specifications related to each method (e.g. range, precision, etc.) refer to the related measurement section.
PRECISION AND ACCURACY
Precision is how closely repeated measurements agree with each other. Precision is usually expressed as standard deviation (SD).
Accuracy is defined as the nearness of a test result to the true value. Although good precision suggests good accuracy, precise results can be inaccurate. The figure explains these definitions. For each method, the precision is expressed in the related measurement section as standard deviation at a specific concentration value of the analite. The standard deviation is obtained with a single instrument using a representative lot of reagents.
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